By analysis of the immunoglobulin and T cell receptor (TCR) gene recombination events and the differentialĮxpression of lymphocyte-specific genes, much has been learned about the regulation of B and T cell maturation ( Clevers et al. The molecular mechanisms by which B and T lymphocytes are generated from hematopoietic stem cells have been the subject of These results establish immunoaffinity chromatographyĪs a useful method for identifying Ikaros partners and suggest that Helios is a limiting regulatory subunit for Ikaros within Unlike the B cell results, however, only a fraction of the Ikaros, presumably the fractionĪssociated with Helios, exhibited centromeric localization in T cells. Interestingly, the Ikaros–Helios complexes localize to the centromeric regions of T cell nuclei, similar to the Ikaros localization ![]() The Ikaros–Helios interaction in T cells is supported by the quantitative association of Helios with a fraction of the Ikaros. Although Ikaros is present in most hematopoietic cells, Helios was found primarily in T cells. HeliosĪnd Ikaros recognize similar DNA sequences and, when overexpressed, Helios associates indiscriminately with the various Ikaros The p70 gene, named Helios, encodes two protein isoforms with zinc finger domains exhibiting considerable homology to those within Ikaros proteins. The complexes from an immature T cell line were purified, revealing associated ![]() To be components of highly stable complexes. In this study, Ikaros proteins were found That Ikaros proteins colocalize with inactive genes and centromeric heterochromatin. The intracellular functions of Ikaros are not known, although recent studies have shown The Ikaros gene encodes multiple protein isoforms that contribute critical functions during the development of lymphocytesĪnd other hematopoietic cell types.
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